Sample Collection - Chytrid Testing

Follow the guidelines below for step by step instructions to swab and send samples for Batrachochytrium dendrobatidis (Chytrid) testing.

• Using a plain swab (i.e. no transport medium), wipe the underside of the legs, feet and drink patch 3-5 times each, with the same swab tip.
  • There is limited published evidence that fine tip rayon tipped swabs (e.g. Medical Wire & Equipment Co. MW 100–100) are most sensitive for DNA recovery (Boyle et al, 2007*). These are available through IZVG if required, and if the testing process can be delayed whilst these are ordered and sent out.
• For screening each frog, use one swab per frog. Identify each swab/frog separately.
• For screening a group, either use the same single plain swab on several frogs, or use a different swab tip on each frog, and then send all together in one pot per group (with a request for pool testing).
• Plain swabs should be sent by routine post at room temperature in green boxes or other packaging, to the usual Station House address.
• Use a standard IZVG Pathology aquarium or zoo submission form and write “Chytrid PCR test” in the OTHER box.
• It is advised to pack swab samples separately from formalin-fixed tissues, as formalin fumes/spillage can damage the nucleic acids on the swabs and reduce PCR sensitivity.

Notes:
*A protocol for chytrid sampling is described and illustrated in : Hyatt, A.D., Boyle, A. H. D., Olsen, V., Boyle, D. B., Berger, L., Obendorf, D., Dalton, A., ... & Campbell, R. (2007). Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis. Diseases of aquatic organisms, 73(3), 175-192. (available on request)

SUBMISSION FORM AQUATIC SUBMISSION FORM

If you have any queries about specific tests or sampling, please contact the pathologists or ring 01535 692000.